The Group II Chaperonin Mm-Cpn Binds and Refolds Human Gamma D Crystallin

Crystal structure of group II chaperonin in the open state.

Thermosomes are group II chaperonins responsible for protein refolding in an ATP-dependent manner. Little is known regarding the conformational changes of thermosomes during their functional cycle due to a lack of high-resolution structure in the open state. Here, we report the first complete crystal structure of thermosome (rATcpnβ) in the open state from Acidianus tengchongensis. There is a ∼...

"Folding, Unfolding and TriC Chaperonin Recognition of Human γD- Crystallin in Relation to Cataract"

Decrease in protein solubility and cataract formation caused by the Pro23 to Thr mutation in human gamma D-crystallin.

The P23T mutation in the human gammaD-crystallin gene has in recent years been associated with a number of well known cataract phenotypes. To understand the molecular mechanism of lens opacity caused by this mutation, we expressed human gammaD-crystallin (HGD), the P23T mutant, and other related mutant proteins in Escherichia coli and compared the structures and thermodynamic properties of thes...

Cloning, structure and mRNA expression of human Cctg, which encodes the chaperonin subunit CCT gamma.

We describe the cloning, DNA sequence analysis and mRNA expression analysis of human Cctg (HsCctg), a gene that encodes the gamma-subunit of the eukaryotic cytosolic 'chaperonin-containing TCP-1' (CCT). Partial clones representing the 3' region of HsCctg cDNA were isolated from a human kidney cDNA library, and the missing 5' region was amplified directly from human kidney cDNA. The Cctg mRNA tr...

Comparison of human MM, NN, and MN blood group antigens.

Types MM, NN, and MN blood group substances have been isolated. All three materials are glycoproteins containing about 24 % iV-acetyhreuraminic acid, 10 % galactosamine, 5% glucosamine, and 14% galactose. The hexosamines appear to be N-acetylated. Amino acid analysis of the glycoproteins reveals them to be similar in composition and to be especially rich in serine and threonine. Treatment of th...